2. . The system pressure and PreColumn pressure also fluctuates between 0. Figure 7: Pump tab. Figure 6: Pump head with purge valve and outlet to autosampler. Check for signs of leaks. 1. Purification Solvent Management. 7. download video. ISET enables. Note: Follow the instructions in the Ready Check Results window and close both purge screws. , 5-10 mL/min. Vanquish Binary Pump F: Up to 1000 bar (15,000 psi) and 8 mL/min. Solvent mixing: The proportionating valve (lower-front of image) allows the desired volume ratio of the different solvents to be drawn from the reservoirs. L/mni for 5mni . A purge valve including a PTFE frit is fitted at the pump outlet for convenient priming of the pump head. a. ) If system has check valve, loosen valve to allow air to escape. Initial System inspection It is always worthwhile to spend a few minutes before starting analysis on assessing the system suitability for the analysis task in hand. ) The image on the right shows a fairly typical ternary pump, which can be controlled via either the front panel or computer control. Required parts: New pump head; Spacing tool; Size 6 hexagonal wrench; ¼” wrench; Preparation steps: Flush the pump head with an appropriate solvent using the purge function Next, it purges the flow lines up to the column with the mobile phase at the starting gradient concentration and then pumps it through the column at a low flow rate to protect the column. The table below describes the material covered in this book. Purge Valve Assembly: 1 - Valve body, 2 - PTFE frit with groove, 3 - Seal cap. 400 bar max. pump program to 90/10 water/ACN to flush the column for 15 min at 1. 8 Click pump on the flow diagram and change the flow path into A2 and B2. HPLC maintenance tutorial on how to change the High Pressure Filter on an Agilent 1290 G4220x or G4204x Pump. You may use the. Remove sample vials from the auto-sampler tray g. Consumables likely need to be replaced on aqueous pump. Residues or contaminations may block filters or capillaries. ⬇️ Get Affordable and Dope Lab Consumables Here ⬇️(No pun intended, unless you're a cannabis lab, then pun intended)to Connect with. g. BTW: I find it useful to tip the flat bottomed solvent. From my little experience i learnt that it may be due to air trapped in. Perform search. Initially, the pressure was unstable. Press the EDIT/ ENTER key. The 515 HPLC pump is a programmable, free-standing HPLC pump available in isocratic, gradient, and semi-preparative configurations: Precise stepper motor control (48 steps/µL resolution) of dual reciprocating pistons. The system pressure and PreColumn pressure also fluctuates between 0. solvents. 7. Purge and condition lines A1, B1 using pathway 1, then A2, B2 using pathway 2 using a 50:50 composition to ensure both pumps are taking and delivering the same volume of HPLC flushing solvent. If only an occasional bubble is present, the flow rate will be erratic, causing retention time problems. Purge Valve Assembly 115 Active Inlet Valve Assembly 116 Accessory Kit G1311-68705 117. With an unrivaled flow-pressure footprint, the RSLC pump is the most flexible binary UHPLC pump available. Liquid Chromatography. Flush the pump with 100% filtered, distilled water for at least 3 minutes. Purge valve: Reciprocating piston pumps do not work if there is air in the lines, which can be the case after changing a solvent reservoir, or leaving the pump off. • Purge each channe lwtih 25–3m. An autosampler works by aspirating and injecting a precise sample volume into the mobile phase stream. 2. Purge the mobile phase reservoir by opening the purging valve. 05 min is normal however the behavior of the method should be used to determine what is a normal variation. • Flush the entire system flow path with water for at least 10 min at 2 mL/min. - after changing solvents. Operable in constant flow or constant pressure mode, as well as isocratic, low pressure gradient, ternary high-pressure gradient, or quaternary low. 5 0. Place the solvent bottle back into LC eluent. The Agilent 1260 Infinity II Flexible Pump is a quaternary UHPLC system pump that provides gradient formation and solvent blending from up to four solvents. On – line vacuum degassing is available on most commercial available systems. 8:42. A pump influence is indicated by baseline fluctuations synchronous with the pump cycle. DO NOT PURGE THE SYSTEM WITH THE BALL VALVE CLOSED! THIS WILL RESULT IN PRESSURE TOO HIGH FOR THE PUMP TO HANDLE AND CAN RUIN THE COLUMN!!! 6. Consumables likely need to be replaced on aqueous pump. Draw approximately 20 mL of fluid. 05 0 0. (Prime each. More lab efficiency with your auto delivery schedule, modify and cancel it. 000, Ready, 0. Purge the Pump (whenever changing buffers) a. If still you have the same problem, checkout the rest of tubing. After waiting a specified time, it starts pumping the mobile phase at the flow rate specified in the analytical method to automatically equilibrate the. If the pressure reaches its maximum (press. Always do this one channel at a time (e. , 5-10 mL/min. 11 Click everything on. 5:26. 8 um columns. However, even if the degassing system works properly, some air bubbles might stick in solvent lines or U/HPLC system fluidics. Air bubbles Filter using a 0. From my little experience i learnt that it may be due to air trapped in. So for example, if you normally run at 100 bar and the pressure drops to 5 bar, then the pump will shut down. Check that appropriate mixingThe 322 HPLC Pump is a multi-solvent pump for analytical to semi-preparative high performance liquid chromatography (HPLC). close the purge. I am working with HPLC Shimadzu 20AD fitted with LC20AD. Purge pump at high flow rate (e. 3 Crucial Points to achieve ultimate quaternary pump performance (low pressure mixing!): 1. 6:51. Replace the pump head if the wetted surface is damaged, resulting in internal leaks compromising pump performance or if the pressure sensor is faulty. the purge valve, and the actual pump mechanism. On the front panel of the pumps, press the VP button. Varian Modular Analytical HPLC Systems Figure 1. Purging air from pump system: Change solvent line to a bottle containing 100% isopropanol. United States. 45 µm inert hydrophilic filter (avoid cellulose) and degas all eluents thoroughly, using sonication under vacuum. 2. In the right part of the image, the glass piston is. Put the Pump A in your buffer. If problem persists, flush system with 100% methanol or isopropanol. Set the flow rate to 5 ml/min d. Check the solvent line filters for permeability. g. 물론 이건 내 생각이다! 여튼 purge. 1. For. Basic HPLC System Components. a Continue to purge for a short while. Set a flow of 5 ml/min and purge each channel for 5 min with HPLC-quality Water. The pressure can be monitored at your analog output signal, with the handheld controller,With the Waters Arc HPLC System, you can count on high-efficiency separations and quality data to meet your regulatory requirements with confidence. 1 Purpose 4 1. 05 0 0. 02-0. The degasser is a standard feature of the 1525µ Binary HPLC Pump and an optional feature of the 1525 analytical and 1525 EF Binary HPLC Pumps. With Arc HPLC System, you can easily replicate and improve the performance of your existing LC methods without compromising data quality eliminating the burdens of older, less efficient LC systems. The pump assembly includes a pump head with an active inlet valve which has an replaceable cartridge, and an outlet valve. derivatization reagent, microdialysis liquid,…)The 1290 Infinity II Bio Flexible Pump is a UHPLC pump consisting of biocompatible material, designed for use in biopharma and other applications utilizing high-salt and extreme-pH conditions. The purification takes place in a separation column between a stationary and a mobile phase. 2. Replacing a Check Valve Cartridge. What is purging in HPLC? Answer: “When setting up an HPLC system, the aim of the purge is simply to flush through all the lines so that any remaining solvent in them from a previous analysis or wash is replaced with the new mobile phase. . 1 Calibration of the Pump is to be done with the help of distilled water at 25°C±2°C. The system must be primed to remove air bubbles and for the pump to function properly. Is the pump fuse in working order?Discover faster analysis, better resolution, and more reproducible chromatography with our new flagship UHPLC pump. So, let's get started and purge away any confusion! Purging, in the context of lab equipment, is the process of flushing a lot of. I usually let the column drip into a beaker while washing it. 3. In general, the degasser should be left on, and all solvent lines including the unused ones should be filled with solvents. We have been experiencing some pressure problems on our hplc lately and the problem is mostly fluctuations in the pump head. 3. In such case clogging of the column is most likely happening due to: additive (i. It offers gradient formation from up to four solvents at pressures up to 1300 bar and flow rates up to 5 mL/min. 6. Santa Clara, CA 95051. If seal wash option is installed, replace wash seals and gaskets. 0:44. Perform flow rate check. 1260 Infinity Binary Pump User Manual 11 Introduction to the Binary Pump 1 Instrument and OperationThe pump assembly includes a pump head with a passive inlet valve and an outlet valve. 0 hours and also use 50% Methanol after water flushing. You may find that the manufacturer of your particular. If using MassLynx or Empower. - after changing solvents. Each channel comprises a pump assembly including pump drive, pump head, active inlet valve with replaceable cartridge, and outlet valve. Priming and Purging the 1525 Binary HPLC Pump. The PTFE frit is very accessible and easy to switch out, too. The following diagrams illustrate the operating principle of a modern HPLC pump: an electric motor drives a piston (generally made of sapphire or ceramic material) in and out of metal, ceramic, or PEEK piston chamber. Once the air is removed, close the valve and start flow. 00 ml/min, turn the pump back ON and close the prime purge valve to allow the liquid to flow through solvent lines, through the degasser channel, through the pump. The pump inlet port is closed to allow the pump to run with the gas ballast valve in the open position, which helps to purge the pump. Mobile phase is passed through porous polymer tubing placed in a vacuum chamber. Change the gradient. Flow anomalies can interfere with the chromatography and stand in the way of repeatable results. Our industry leading LC-40D X3 pump ensures precision and accuracy at all flow rates. 9 Purge a short while longer, and then close the purge valve. Press the EDIT/ ENTER key. This chemical treatment process leads to a protective oxide. As well as excellent accuracy, it has very small delay volumes of ≤ 350 μL. For more information, visit tutorial demonstrates how to prime the pumps in Bio-Rad's new NGC liquid chromatography sy. It is by far the number one cause of problems in HPLC. - filter non-HPLC grade solvents - filter buffer solutions - Install an in-line filter between auto-sampler and column (removes pump seal debris, ALS rotor debris, and sample particulates). Then press “Enter”, “1”, “Enter”, “CE”, “CE”. 1 Turn on LC20AD (component 4) and SIL20A (component 3). Purge pump at high flow rate (e. 1, 3, and 4. HPLC Principle. Agilent produces a variety of inlet and outlet valves, manufactured to Agilent's high standards for quality, security, and integrity, and designed to ensure the ongoing stability and performance of pump systems in LC applications. b. When I used the pump C last week the pressure suddenly fell down from 300 PSI to 0 (with 100% methanol, 0. 2HPLC is a High Performance liquid Chromatography. Power off the pump and remove the waste tubing from the purge valve. Fig 2. Excellent gradient performance resulting from the highly accurate solvent delivery by the 5110/5160 pump with Hitachi’s unique high-speed. Blockage of. Vent. 02 in. • Syringe, loop & injector plunger seal. High Performance Liquid Chromatography (HPLC) is an analytical technique that is ubiquitous in labs across the globe. , 5-10 mL/min. Purge Line Figure 1 322 HPLC Pump. How do i manually prime/purge it? Because there are some air bubbles, and if they get inside the needle, its gonna be bad news bears ( i think). Turn off the pump flow and wait until the system pressure is down to zero. Label bottles correctly with bottle content, and filling date / expiry date. The 1260 Infinity II Quaternary Pump combines proven reliability with highest flexibility in automated. The 1260 Infinity II Flexible Pump is a quaternary UHPLC pump that provides gradient formation and solvent blending from up to four solvents. 1. From my little experience i learnt that it may be due to air trapped in. Retention. Fig. 6. Fig. The system must be primed to remove air bubbles and for the pump to function properly. If problem still persists, contact system manufacturer. Rugged and dependable, Arc HPLC System delivers the ideal balance of performance and value for right-the-first-time analysis. This video demonstrates the proper procedure to manually prime the pump on a Shimadzu LC 40 instrument. Prepare the solvent: Begin by ensuring that the solvent is filtered and degassed. What is pump seal in HPLC?Dionex UltiMate 3000 2G Pump Maintenance Kits vary by pump. Plot the graph of Injection volume against area. 06% or 0. 3. Remove and disassemble the pump head. Run at least 25 ml buffer through degasser and pump (5 minutes) e. Remove and disassemble the pump head. High Priced Liquid Chromatography. "Chromatography" is a technique for separation, "chromatogram" is the result of chromatography, and "chromatograph" is the instrument used to conduct chromatography. Press the EDIT/ ENTER key to start purging. During step 1, check that the solvent is freely moving through the solvent lines. Wash the system with isopropanol (excluding the column). closed. 1–1% solution by volume is sufficient. The pressure does not rise. Press the EDIT/ ENTER key to start purging. categories. 1) Locate the pump draw-off valce (handle located on front of the pump) and priming syringe. Purging the solvent pumps 1. Select this procedure when you want to prime and purge any 1525 Binary Series Pump. g. Easy to use fraction collector and Versatile Liquid Handler. The ultra-high pressure capacity (up to 130 MPa/1300 bar) allows the of sub-2μm particle columns for high efficiency chromatography, especially for LC. If tiny air bubbles are observed in the lines continuously, it’s. Use 2 um frit for 3. The two channels are fed into a low-volume mixing chamber which is connected via a restriction capillary coil to a damping unit and a mixer. 1290 Infinity II High Speed Pump. Proper storage of HPLC columns will increase longevity and performance. Pump 15 min at this pressure to wear the seals in. duration. Purge the pump, check general functionality (for instance with a diagnostics test), read pre-compression value from pump panel and compare against values from manual (Thermo Scientific Dionex pumps only). The purge valve, acting as an essential component of your vehicle’s Evaporative Emissions Control System (EVAP), plays a vital role in reducing harmful emissions and maintaining fuel efficiency. The easy to use and economically designed LC-40D pump enables reliable HPLC chromatography in isocratic, binary, or LPGE modes. Open the "drain" on LC-20AD. Open the black purge valve and press the “Purge” button. 2. Pressure B. Use the keypad to type in the solvent composition for pumps A, B, C, and D. In a low-pressure gradient (LPG) pump type, a proportioning valve is used to aspirate the desired solvent mix. Filter all samples and standards 3. However, in order to obtain a full understanding of the pump, Dionex recommends that you review the manual thoroughly before beginning operation. The target url is a user manual for the Agilent 1290 Infinity Quaternary Pump G4204A, a high-performance liquid chromatography (HPLC) device that can handle a wide range of flow rates and compositions. Loaded 0%. PROCEDURE: Flush the system using one of the cleaning mixtures as described in the cleaning. Gradually increase system flow up to the required flow rate,. Click the [Auto Purge] button to perform auto purge. c Enter the new parameters in step 8, and click OK. Solving Problems That Matter - Bioprocessing. Perform sample clean-up (i. keep the system at this flow rate for about 10 minutes to equilibrate the system without the Column. Disconnect the line leading from the column to the detector. When the purge valve is open, solvent from the quaternary pump will go straight to waste rather than proceeding through the column. Waters First Quarter 2023 Highlights. Calculate correlation coefficient should not be less than 0. 2. This will redirect the solvent flow to the waste exit of the valve instead of passing through the. g. 2 Wellplate Autosampler Purge Kit Features Features The new Wellplate Autosampler Purge Kit offers the following features: • Fits for Agilent 1100 Series Wellplate Autosampler (G1367A and G1368A (thermostatted)). That could help to reduce air bubble generation. Column frits are a common point of clogging. Worldwide Numbers . Overall Routine Pump Maintenance 1. Pump 15 minutes at this pressure to wear in the seals. Purge HPLC pumps with buffer made in step 1. 5 um frit for 1. If the solvent line is "dry," perform "dry" primes by opening the manual prime and purge valve, and draw out 10 mL from each solvent line using a syringe. The reduction in condensation risk can allow the pump to achieve its vapor duty at. The target url is a user manual for the Agilent 1290 Infinity Quaternary Pump G4204A, a high-performance liquid chromatography (HPLC) device that can handle a wide range of flow rates and compositions. First, initially raise the helium flow_rate (adjusting it via pressure to 0. This is needed to purge any air from the tubing, introduce fresh mobile phase to. Select this procedure when you want to prime and purge any 1525 Binary Series. This pump enables quaternary solvent delivery at pressures up to 800. Start pump purging (all channels, including those not used for the application) and wait until pump purging is complete; Flush the entire chromatography system with the starting. 2 μm filters. Liquid Chromatography (LC) Troubleshooting Resources. Figure 1 Overview of the binary pump Purge valve Active inlet valve Mixer Outlet valve. Two of the most widely used methods to degas a mobile phase are: Helium purging: helium is bubbled through the solvent and removes up to 80% of dissolved air. . (Prime each pump head separately. 5 01. When performing a purge injector function on a 2695 module, where is the solvent. OBJECTIVE: To clean and flush an Alliance HPLC System or an ACQUITY UPLC system to remove contaminants. The FRC-40 features an open-access design, a parameter simulation to ensure pure fractions, compatibility with many racks, and up to 4-channel acquisition. Open the Purge Valve of your pump and purge each solvent line, one by one, with HPLC-quality Isopropanol (IPA). 9 Pump Types LPG pump type Eluent composition at the low pressure side => Before the pump head Eluent is composed through proportioning valves Eluent segments pass working and equilibration cylinder Up to four solvents can be mixed HPG pump type Eluent composition at the high pressure side => Behind the pump head Pure solvents pass. 000 ml, – 25 psi. Next, it purges the flow lines up to the column with the mobile phase at the starting gradient concentration and then pumps it through the column at a low flow rate to protect the column. Using an autosampler reduces manual labor and improves injection precision and accuracy compared to manual sample injection, an absolute priority for a quantitative HPLC analysis. , 10 mL/min) to clear all the air from the pump and connecting tubing. See "Pressure pulsation or inconstant pressure" in Pressure on page 120. With the Arc HPLC, you can easily replicate and improve the performance of. Overlay of 6 pump proportioning chromatograms in 10% gradient. Agilent University. Replacing the Plunger Seal and Plunger. 7. Click the [Auto Purge] button to perform auto purge. Change the pump program back to 50/50 water/ACN to re-equilibrate the system e. Set the pump to 100% 'A' at 1. UltiMate 3000 Series: SD, RS, BM, and BX Pumps Operating Instructions Page I Declaration of Conformity (Original Declaration of Conformity) Product: Thermo Scientific Dionex UltiMate 3000 - Pump Types: ISO-3100SD, ISO-3100BMWe have been experiencing some pressure problems on our hplc lately and the problem is mostly fluctuations in the pump head. 322 HPLC PumP | uSER’S GuIDE 13 De SC r I pt ION pUMp heaDS Two interchangeable pump head types are available:Passivation is a chemical treatment of stainless steel and purging is a cleaning process. The binary Semipreparative High-Pressure Gradient P680 Pump is ideal for small-scale purification applications. 5:26. This purging is carried out for several hours or overnight, depending on the system's size and the degree of contamination. If problem. Flush the column with 10/90 water/ACN for 5 min at 1. Chapter/Appendix Description Chapter 1, Overview of the Waters 1500 Series HPLC Pump Describes the. 1. 1 A) Calibration of Pump:-. • Future-proof flow-pressure footprint provides highest application flexibility. operating pressure (9,99 ml/min max. However, in order to obtain a full understanding of the pump, Thermo Fisher Scientific recommends that you review the manual thoroughly before beginning operation. Run each pump for 2 minutes. degasser ports and/or pump head multichannel valve or pump head inlet, as needed, to draw liquid through until all lines are filled. • Mast kit with mixer, purge valves and manual injector • Pre-formed 0. Make sure to confirm that the purge valve isHow to prevent your HPLC from running dry. This is needed to purge any air from the tubing, introduce fresh mobile phase to each line and then toHPLC, and biocompatible pumps. closed. Low delay volume, extended pressure range up to 800 bar, analytical flow rates up to 5. g. Among the various technologies developed for chromatography,. Remove and clean pistons. 2. Program the pump to purge the column with strong solvents. 이동상을 준비하고 pump를 ON하기 전에 purge를 실시하는데, 3분 정도 purge를 하도록 설정돼있습니다. 1) Locate the pump draw-off valce (handle located on front of the pump) and priming syringe. A = 100%). Discover the efficient LC-40D pump for routine HPLC applications up to 44 MPa (440 bar. Monitoring your HPLC system for potential issues can help to. 0*Steps to follow: To backflush the needle seat according to the following procedure, you need a pump with a manual purge valve. Inside the purge valve is a small piece of polytetrafluoroethylene, called a PTFE frit. When they fill with air, they lose their prime and stop • Effects Column Life pumping [26]. Flexible Pump The Agilent 1290 Infinity II Flexible Pump improves your efficiency by combining the performance of a high-pressure mixing UHPLC pump with the flexibility of a low-pressure mixing UHPLC pump. HPLC Pump: The HPLC pump produces high pressure that gives a continuous and reproducible flow to the mobile phase throughout the HPLC system. Sonication: ultrasonic baths as a stand-alone. RCM (right click mouse) in the Quat Pump module i. If you've ever wondered how to purge this piece of equipment, you're in the right place. Set the maximum flow gradient to 100 ml/min2 c. The 1260 Infinity II Quaternary Pump is a gradient pump that uses low-pressure mixing for UHPLC gradient formation and solvent blending from up to four solvents. Eluent may then be allowed to progress through the IC system, and the pressure monitored. Flush the pump with 100% filtered, distilled water for at least 3 minutes. g. Then use your nomal mobil phase. ID stainless steel tubing kit • Galaxie Chromatography Software-0. The reduction in condensation risk can allow the pump to achieve its vapor duty at. Pump Injector/Autosampler Column Detector Data System/Integrator HPLC System Components Problems Can Be Related to All Components in the System Page 4 Categories of Column and System Problems A. Algae Growth in HPLC Systems 54 Prevent Blocking of Solvent Filters 55 Normal Phase Applications 57 4 Optimizing Performance 59. Algae Growth in HPLC Systems 81 Prevent Blocking of Solvent Filters 82 Normal Phase Applications 83 4 Optimizing Performance 85 Using the Degasser 86. After the valve sonication procedure, it is suggested to purge the pump with water at 60 degrees, keeping the purge valve open. Steps to follow: Store the column in an appropriate solvent (check column information sheet) When you remove the column, add a column union so the detector gets flushed as well in the following steps. TP01408 RUN STOP EDIT ENTER MENU Waters 515 HPLC Pump Draw-off valve Seal wash hole (not visible) Pump head assembly High. Open the black purge valve on the front of the quaternary pump by turning it several times counterclockwise. The system will start the countdown from 5 minutes to zero. This pump operates at pressures up to 600 bar and flow rates up to 10 mL/min. , 5-10 mL/min. Pump – Open the purge valve and purge every solvent channel with 5 mL/min for 5 minutes to remove bubbles in your solvent lines (Note: if the backpressure at purging is higher than 10 bar, please replace the PTFE frit in the purge valve) – Make sure that the flushing solvent is mixable with the storage solvent of your solvent channel. off. Then wash them out with water and re-assemble them on the pump head. This will redirect the solvent flow to the waste exit of the valve instead of passing through the whole system. 5 um columns, use 0. 3:36. Start pump purging (all channels, including those not used for the application) and wait until pump purging is complete; Flush the entire chromatography system with the starting solvent of your application at a reasonable flow rate to rinse out any other solvent; Install the appropriate HPLC column for your application; Close all doors of the. Close prime/purge valve. Pump & Maintenance Kits for HPLC Part Number: 01018-22707. 17: Ready Check Results window appear s after the NC pump blocks purge is executed 2. 6:51. Contacting Us Vanquish Pumps (VH-P10) Operating Manual Page 3 Contacting Us There are several ways to contact us: Ordering Information For ordering information or sales support for HPLC products, contactSolving Problems That Matter - Batteries. HPLC pumps are designed to pump liquid, not air. |The 1290 Infinity II Bio Flexible Pump. The 1260 Infinity II Binary Pump is ideally suited for a broad range of UHPLC applications using columns with conventional, superficially porous or sub-2-micron particles. 1 Overview of HPLC. Detector – responds to the separated analytes emerging from the HPLC. Press “Prime” button (or pump at max flow), continue to draw on syringe until no bubbles are seen. Agilent recommends a purge of at least 30mL in total for the 1200 systems. Rebuilding the Reference Valve. From my little experience i learnt that it may be due to air trapped in. The pump assembly includes a pump head with an active inlet valve which has an replaceable cartridge, and an outlet valve. Use only HPLC-grade solvents and water filtered through 0. Replace PTFE frit in the purge valve. Turn . Removing dissolved gases from the mobile phase improves the performance andSo that is how you fix an air bubble in the pump. Second, the dissolved gasses must diffuse through the membrane (flat film or tubing wall). Manual - isocratic HPLC System G1380AA standard configuration • G1310A isocratic pump with solvent cabinet • G1328A manual injector (MI) • G1314A variable wavelength detector (VWD) with standard flow cell (10 mm path length, 14 µl volume, 40 bar maximum pressure) • G2220AA 2D-Value Solution ChemStation other modules that can be addedFailure to use fresh clean pickup filters in your HPLC may lead to total contamination of the flow path (column, pump, A/S and detector(s)). 4411-EN Revision 2. HPLC maintenance tutorial on how to remove the pump head on an 1100/1200/1260 Agilent HPLC. 1-15 of 16. Newsroom; Company Information;HPLC Setup Section 6. Pump problem – Contact your maintenance provider.